Technical Data Sheet
Rabbit Anti-Mouse FMC63 scFv Monoclonal Antibody
Product Information
Material Number:
200100
Size:
100 Tests
Vol. per Test:
1 μL
Antibody Types:
Monoclonal
Clone:
R19M
Immunogen:
scFv region of a CD19-specific mouse mAb clone FMC63
Host Species
Rabbit
Reactivity:
Mouse
Storage Buffer:
Aqueous buffered solution containing protein stabilizer and ≤0.03% sodium azide.
Description
The rabbit monoclonal antibody R19M specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.
Flow cytometric analysis of anti-CD19 CAR expression on Jurkat cells. Jurkat cells were lentivirally transduced with anti-CD19 CAR and cultured. 2×105 cells were stained for the expression of anti-CD19 CAR with Rabbit Anti-Mouse FMC63 scFv Monoclonal Antibody (Cat. No. 200100, right panel). Secondary staining was carried out with Goat anti-Rabbit IgG (H+L), Alexa Fluor 647 (Cat. No. 700002). Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel).
Preparation and Storage
Store undiluted at 2-8°C. Do not freeze.
The monoclonal antibody was purified by Protein A.
Application Notes
Application
Flow cytometry
Routinely Tested
Product Notices
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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