Technical Data Sheet
Anti-Mouse FMC63 scFv Recombinant Antibody, hIgGC
Product Information
Material Number:
200819
Concentration:
1.0 mg/mL
Size:
100 mg
Antibody Types:
Primary antibodies, recombinant antibodies
Isotype
Recombinant human IgG1, κlight chain
Clone:
Ra1D6
Immunogen:
scFv region of a CD19-specific mouse mAb clone FMC63
Reactivity:
Mouse
Storage Buffer:
PBS, pH 7.4
Preservative:
None
Description
Ra1D6 specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.
Flow cytometric analysis of anti-CD19 CAR expression on Jurkat cells. Jurkat cells were lentivirally transduced with anti-CD19 CAR and cultured for 7 days. 2×105 cells were stained for the expression of anti-CD19 CAR with Anti-Mouse FMC63 scFv Monoclonal Antibody, hIgGC (Cat. No. 200819, right panel). Secondary staining was carried out with Goat anti-Human IgG+IgM (H+L), Alexa Fluor 647. Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel).
Preparation and Storage
Shipped at 2-8 °C. Store at 2-8 °C short term (2 weeks). Store at -20°C in small aliquots for long term storage. Avoid freeze/thaw cycle.
The monoclonal antibody was purified by affinity chromatography.
Application Notes
Application
Flow cytometry
Routinely Tested
Product Notices
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
