scFv region of a CD19-specific mouse mAb clone FMC63
PBS, pH 7.4
Ra1D6 specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.
Flow cytometric analysis of anti-CD19 CAR expression on Jurkat cells. Jurkat cells were lentivirally transduced with anti-CD19 CAR and cultured for 7 days. 2×105 cells were stained for the expression of anti-CD19 CAR with Anti-Mouse FMC63 scFv Monoclonal Antibody, hIgGC (Cat. No. 200819, right panel).Secondary staining was carried out with Goat anti-Human IgG+IgM (H+L), Alexa Fluor 647. Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel).
Preparation and Storage
Shipped at 2-8 °C. Store at 2-8 °C short term (2 weeks). Store at -20°C in small aliquots for long term storage. Avoid freeze/thaw cycle.
The monoclonal antibody was purified by affinity chromatography.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.