scFv region of a CD19-specific mouse mAb clone FMC63
PBS, pH 7.4 and ≤0.03% sodium azide
The rabbit polyclonal antibody R19P specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.
Flow cytometric analysis of anti-CD19 CAR expression on Jurkat cells. Jurkat cells were lentivirally transduced with anti-CD19 CAR and cultured. 5×105 cells were stained for the expression of anti-CD19 CAR with Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Cat. No. 500019, right panel). Secondary staining was carried out with Goat anti-Rabbit IgG (H+L), Alexa Fluor 647 (Cat. No. 700001). Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel).Acquisition of >10, 000 events was performed.
Preparation and Storage
Shipped at 2-8°C. Store at 2-8°C short term (2 weeks). Store at -20°C in small aliquots for long term storage. Avoid freeze/thaw cycle.
The polyclonal antibody was purified by Protein A.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.