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    • Technical Data Sheet

      Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody

      Product Information

      Material Number:

      500035

      Size:

      1.0 mg (This product has been overfilled to ensure total recovery of stated quantity.)

      Concentration:

      1.0 mg/mL

      Purity:

      >90% by SDS-PAGE

      Antibody Types:

      Polyclonal

      Immunogen:

      scFv region of a CD19-specific mouse mAb clone FMC63

      Reactivity:

      Mouse

      Buffer:

      PBS, pH 7.4

      Storage and Rehydration:

      Store freeze-dried solid at -20°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 2 weeks at 2-8°C as an undiluted liquid.

      Extended Storage after Rehydration:

      Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing.

      Expiration date:

      One year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

      Description

      The rabbit polyclonal antibody R19P specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

      Flow cytometric analysis of anti-CD19 CAR expression on Jurkat cells. Jurkat cells were lentivirally transduced with anti-CD19 CAR and cultured for 7 days. 2×105 cells were stained for the expression of anti-CD19 CAR with Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Cat. No. 500035, right panel). Secondary staining was carried out with Goat anti-Rabbit IgG (H+L), Alexa Fluor 647. Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel). Acquisition of >10, 000 events was performed.

      Application Notes

      Application

         Flow cytometry

         Routinely Tested

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

    BioSwan Laboratories, Co., Ltd.

    Room 808,

    No.781 Cailun Road,

    Pudong New District, Shanghai 201203

    P. R. China

    Customer Service

    Information / Ordering

    86-21-50207339

    info@bioswan.com

    Distributor

    上海星湾生物技术有限公司 Copyright 2019

    沪ICP备2021004258号-1
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