1.0 mg (This product has been overfilled to ensure total recovery of stated quantity.)
>90% by SDS-PAGE
scFv region of a CD19-specific mouse mAb clone FMC63
PBS, pH 7.4
Storage and Rehydration:
Store freeze-dried solid at -20°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 2 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration:
Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing.
One year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
The rabbit polyclonal antibody R19P specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.
Flow cytometric analysis of anti-CD19 CAR expression on Jurkat cells. Jurkat cells were lentivirally transduced with anti-CD19 CAR and cultured for 7 days. 2×105 cells were stained for the expression of anti-CD19 CAR with Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Cat. No. 500035, right panel). Secondary staining was carried out with Goat anti-Rabbit IgG (H+L), Alexa Fluor 647. Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel). Acquisition of >10, 000 events was performed.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.