Product Information
Material Number: | 500035 |
Size: | 1.0 mg (This product has been overfilled to ensure total recovery of stated quantity.) |
Concentration: | 1.0 mg/mL |
Purity: | >90% by SDS-PAGE |
Antibody Types: | Polyclonal |
Immunogen: | scFv region of a CD19-specific mouse mAb clone FMC63 |
Reactivity: | Mouse |
Buffer: | PBS, pH 7.4 |
Storage and Rehydration: | Store freeze-dried solid at -20°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 2 weeks at 2-8°C as an undiluted liquid. |
Extended Storage after Rehydration: | Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. |
Expiration date: | One year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use. |
Description
The rabbit polyclonal antibody R19P specifically binds to the scFv region of a CD19-specific mouse monoclonal antibody (mAb, clone FMC63). CD19 antigen is a B-cell specific cell surface antigen, which is expressed in all B-cell lineage malignancies and normal B-cells. The scFv region of FMC63 has been used to develop CD19-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.
Flow cytometric analysis of anti-CD19 CAR expression on Jurkat cells. Jurkat cells were lentivirally transduced with anti-CD19 CAR and cultured for 7 days. 2×105 cells were stained for the expression of anti-CD19 CAR with Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Cat. No. 500035, right panel). Secondary staining was carried out with Goat anti-Rabbit IgG (H+L), Alexa Fluor 647. Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel). Acquisition of >10, 000 events was performed.
Application Notes
Application
Flow cytometry | Routinely Tested |
Product Notices
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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