thumbnail image
  • 首页
  • 走进星湾 
    • 企业文化
    • 荣誉资质
    • 合作伙伴
  • 产品中心 
    • 产品优势
    • 产品目录
  • 资讯中心 
    • 行业动态
    • 公司新闻
  • 联系我们 
    • 联系方式
    • 申请试用
  • 海外经销商 
    • CytoArt, Inc.
    • Shigematsu&Co.,Ltd.
  • …  
    • 首页
    • 走进星湾 
      • 企业文化
      • 荣誉资质
      • 合作伙伴
    • 产品中心 
      • 产品优势
      • 产品目录
    • 资讯中心 
      • 行业动态
      • 公司新闻
    • 联系我们 
      • 联系方式
      • 申请试用
    • 海外经销商 
      • CytoArt, Inc.
      • Shigematsu&Co.,Ltd.
  • 首页
  • 走进星湾 
    • 企业文化
    • 荣誉资质
    • 合作伙伴
  • 产品中心 
    • 产品优势
    • 产品目录
  • 资讯中心 
    • 行业动态
    • 公司新闻
  • 联系我们 
    • 联系方式
    • 申请试用
  • 海外经销商 
    • CytoArt, Inc.
    • Shigematsu&Co.,Ltd.
  • …  
    • 首页
    • 走进星湾 
      • 企业文化
      • 荣誉资质
      • 合作伙伴
    • 产品中心 
      • 产品优势
      • 产品目录
    • 资讯中心 
      • 行业动态
      • 公司新闻
    • 联系我们 
      • 联系方式
      • 申请试用
    • 海外经销商 
      • CytoArt, Inc.
      • Shigematsu&Co.,Ltd.
  • 首页
  • 走进星湾
    • 企业文化
    • 荣誉资质
    • 合作伙伴
  • 产品中心
    • 产品优势
    • 产品目录
  • 资讯中心
    • 行业动态
    • 公司新闻
  • 联系我们
    • 联系方式
    • 申请试用
  • 海外经销商
    • CytoArt, Inc.
    • Shigematsu&Co.,Ltd.
    • Technical Data Sheet

      Rabbit Anti-Mouse C11D5.3 scFv Polyclonal Antibody, Biotin

      Product Information

      Material Number:

      500513

      Size:

      25 Tests

      Vol. per Test:

      1 mL

      Antibody Types:

      Polyclonal

      Immunogen:

      scFv region of mouse mAb clone C11D5.3

      Host Species:

      Rabbit

      Reactivity:

      Mouse

      Storage Buffer:

      Aqueous buffered solution containing protein stabilizer and ≤0.03% sodium azide

      Description

      The rabbit polyclonal antibody BCMA specifically binds to the scFv region of a B-cell maturation antigen (BCMA) specific mouse monoclonal antibody (mAb, clone C11D5.3). B-cell maturation antigen (BCMA) is a protein that has been reported to be selectively expressed by B-lineage cells including multiple myeloma cells and plasma cells. The scFv region of C11D5.3 has been used to develop BCMA-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.

      Preparation and Storage

      Shipped at 2-8°C. Store at 2-8°C. Avoid freeze/thaw cycle.

      The polyclonal antibody was purified by Protein A.

      Application Notes

      Application

         Flow cytometry

         Routinely Tested

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

      FACS Protocol

      1. Harvest the cells and wash the cells once by FACS buffer (PBS containing 2% of BSA).

      2. Count the cells number and the viability, aliquot up to 2×105 live cells into each tube. (Note: the cell viability must be ≥ 95%.)

      3. Wash the cells once by FACS buffer.

      4. Resuspend cells in 100 mL of diluted Rabbit Anti-Mouse C11D5.3 scFv Polyclonal Antibody, Biotin (Cat. No. 500513, 1:100 diluted in FACS buffer) for 30 min at 4°C.

      5. Wash the cells once by FACS buffer.

      6. Resuspend cells in 100 mL of diluted Streptavidin PE (Cat. No. 700032, 1:200 diluted in FACS buffer) for 30 min at 4°C.

      7. Wash the cells 3 times by FACS buffer and resuspend the cells in 200 mL PBS per sample.

      8. Transfer the cells into flow tube and analyze on Flow Cytometer. Acquisition of >10, 000 events is performed.

    BioSwan Laboratories, Co., Ltd.

    Room 808,

    No.781 Cailun Road,

    Pudong New District, Shanghai 201203

    P. R. China

    Customer Service

    Information / Ordering

    86-21-50207339

    info@bioswan.com

    Distributor

    上海星湾生物技术有限公司 Copyright 2019

    沪ICP备2021004258号-1
      星湾生物,精准检测助力更好的CAR-T治疗
      星湾生物是国内首家专注CAR-T细胞精准检测产品与整体解决方案的创新企业。
      https://user-assets.sxlcdn.com/images/572832/FhAedukyC3QpGP5I-A9bu-WoNBdm.png?imageMogr2/strip/auto-orient/thumbnail/1200x630>/quality/90!/format/png
      Cookie的使用
      我们使用cookies来确保流畅的浏览体验。若继续,我们认为你接受使用cookies。
      了解更多