The rabbit polyclonal antibody BCMA specifically binds to the scFv region of a B-cell maturation antigen (BCMA) specific mouse monoclonal antibody (mAb, clone C11D5.3). B-cell maturation antigen (BCMA) is a protein that has been reported to be selectively expressed by B-lineage cells including multiple myeloma cells and plasma cells. The scFv region of C11D5.3 has been used to develop BCMA-specific chimeric antigen receptor (CAR) T cells utilized in clinical trials.
Flow cytometric analysis of anti-BCMA CAR expression on Jurkat cells. Jurkat cells were lentivirally transduced with anti-BCMA CAR and cultured. 5×105 cells were stained for the expression of anti-BCMA CAR with Rabbit Anti-Mouse C11D5.3 scFv Polyclonal Antibody (Cat. No. 500519, right panel). Secondary staining was carried out with Goat anti-Rabbit IgG (H+L), Alexa Fluor 647(Cat. No. 700002). Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel).Acquisition of >10, 000 events was performed.
Preparation and Storage
Shipped at 2-8°C. Store at 2-8°C. Avoid freeze/thaw cycle.
The polyclonal antibody was purified by Protein A.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.