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    • Technical Data Sheet

      AffiniPure F(ab’)2 Fragment Goat anti-Rabbit IgG(H+L), Alexa Fluor 647

      Product Information

      Material Number:

      700002

      Size:

      100 mL

      Concentration:

      0.2 mg/mL

      Host:

      Goat

      Target:

      Excitation laser

      Rabbit

      Red laser(647nm)

      Storage Buffer:

      Aqueous buffered solution containing protein stabilizer and ≤0.03% sodium azide.

      Background

      To minimize cross-reactivity, these goat anti-rabbit IgG whole antibodies have been cross-adsorbed against bovine IgG, goat IgG, mouse IgG, rat IgG, and human IgG. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins.

      Flow cytometric analysis of anti-CD19 CAR expression on Jurkat cells. Jurkat cells were lentivirally transduced with anti-CD19 CAR and cultured. 5×105 cells were stained for the expression of anti-CD19 CAR with Rabbit Anti-Mouse FMC63 scFv Polyclonal Antibody (Cat. No. 500019, right panel). Secondary staining was carried out with AffiniPure F(ab’)2 Fragment Goat anti-Rabbit IgG(H+L), Alexa Fluor 647 (Cat. No. 700002). Non-transduced Jurkat cells were used as a control for gating of CAR expression (left panel).Acquisition of >10, 000 events was performed.

      Preparation and Storage

      Shipped at 2-8°C. Store undiluted at 2-8°C short term (4 weeks) and protected from prolonged exposure to light. Store at -20°C in small aliquots for long term storage. Avoid freeze/thaw cycle.

      Application Notes

      Application

         Flow cytometry

         Routinely Tested

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

    BioSwan Laboratories, Co., Ltd.

    Room 808,

    No.781 Cailun Road,

    Pudong New District, Shanghai 201203

    P. R. China

    Customer Service

    Information / Ordering

    86-21-50207339

    info@bioswan.com

    Distributor

    上海星湾生物技术有限公司 Copyright 2019

    沪ICP备2021004258号-1
      星湾生物,精准检测助力更好的CAR-T治疗
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